基本信息
文件名称：IBA Lifesciences 6xHistidine-tag 使用手册说明书.pdf
文件大小：760.72 KB
总页数：26 页
更新时间：2025-05-17
总字数：约5.63万字
文档摘要

Expressionandpurificationofproteins

using6xHistidine-tag

Acomprehensivemanual

Lastdateofrevision

August2021

VersionPR43-0003

Forresearchuseonly

Importantlicensinginformation

Productsfeaturing“Twin-Strep-tag?”,“Strep-Tactin?XT”arebasedontechnologiescoveredbyintellectualproperty(IP)rights.Oncompletionofthesale,IBA

LifesciencesgrantsrespectiveLimitedUseLabelLicensestopurchaser.IPrightsandLimitedUseLabelLicensesforsaidtechnologiesarefurtherdescribedand

identifiedat/patents-licenses-trademarks.htmloruponinquiryatinfo@oratIBALifesciencesGmbH,

Rudolf-Wissell-Str.28,37079Goettingen,Germany.ByuseofthisproductthepurchaseracceptsthetermsandconditionsofallapplicableLimitedUseLabel

Licenses.

Trademarkinformation

Theownersoftrademarksmarkedby“?”or“TM”areidentifiedat/patents-licenses-trademarks.html.Registerednames,

trademarks,etc.usedinthisdocument,evenwhennotspecificallymarkedassuch,arenottobeconsideredunprotectedbylaw.

Content

Content

1Introduction4

1.1His-tag/Ni-NTAsystem4

1.2Ni-NTAResins4

2Expression9

2.1ExpressioninE.coliwiththetet-system(pASG-IBAvectors)9

2.2Expressionwi