StandardOperatingProcedures
for
FungalIdentification
and
DetectionofAntifungalResistance
AntimicrobialResistanceSurveillanceandResearchNetwork
2ndEdition,2019
IndianCouncilofMedicalResearch
NewDelhi,India
Prof.BalramBhargava
Secretary,DepartmentofHealthResearch(DHR)and
DirectorGeneral,IndianCouncilofMedicalResearch
Publishedin2015,1stEdition
2019,2ndEdition
Printedin2019
Dr.NeerajTandon:Head(PublicationInformation)
PublishedbytheDivisionofPublicationandInformationonbehalfoftheSecretary,
DHRandDirectorGeneral,ICMR,NewDelhi
PrintedbyM/s.RoyalOffsetPrinters,A-89/1,NarainaIndustrialArea,Phase-I,NewDelhi-110028
Foreword
ThefungalinfectionshavebecomeimportanttoIndiancontext,andthismanualdetailstheprocedures
ofidentificationanddetectionofantifungalresistanceformedicallyimportantfungi.ThisStandard
OperatingProcedures(SOP)isintendedtoassistasareferencematerialforroutineactivitiesofa
clinicalmycologylaboratoryofICMRAntimicrobialResistanceSurveillanceandResearchNetwork
(AMRSN).ThiseditionofSOPembodiesthecontinuedeffortsofICMRtoprovideclinicallyrelevant
andpracticaldocumentforthestandardizedinvitrosusceptibilitytestingoffungalpathogens.
Importanttopicssuchasproceduresforthesamplecollection,transportandprocessingofspecimens,
taxonomyandcharacteristicsofimportantfungi,andtechniquesforthecultureandidentification
offungalpathogens,antifungalsusceptibilitytesting(AFST)areelaboratedintheSOP.Thesecond
editionofSOPisupdatedwiththenewtopicssuchasculturalandmicroscopicfeatures(phenotypic
identification)of