棒状杆菌与肉芽肿性乳腺炎讲者：大V
1MicrobiologyLaboratoryand2AnatomicPathologyLaboratory,LabPlus,AucklandHospital,3MolecularMedicineDepartment,FacultyofMedicineandHealthSciences,UniversityofAuckland,and4MicrobiologyLaboratory,MiddlemoreHospital,Auckland,NewZealand
Methods.Womenwithmastitisfromwhomcorynebac-teriawereisolatedfrombreasttissue,aspiratedpus,ordeepwoundswabsin1994–2000wereidentified,andtheircaserecordswerereviewed.Isolatesretrievablefromstockcultureswereidentifiedbyfullbiochemicaltesting,includingnitratereduction,hydrolysisofureaandesculin,fermentationofsugars,andtestingforpyrazinamidase,alkalinephosphatase,andlipophilia.NumericalprofileswereobtainedusingtheAPICORYNEkit(bioMe′rieux).Identificationwasconfirmedby16SrRNAgenesequencingandcomparisonwithsequencedataobtainedfromGenBank,andidentificationwasverifiedby2otherlaboratories.Allbreasthistologicalfindingswerereviewed.
21-55岁平均31岁
结核硬脂酸杆菌Corynebacteriawereisolatedfrombreasttissue,pus,ordeepwoundswabsof24women;themostcommonspeciesisolatedwasthenewlydescribedCorynebacteriumkroppenstedtii,followedbyCorynebacteriumamycolatumandCorynebacteriumtuberculostearicum.Gram-positivebacilliwereseeninsamplessentforcultureorinhistologicalspecimensfor12women,and9ofthe12womenfromwhomadequatehistologicalspecimenswereobtainedhadconditionsthatmetthecriteriaforgranulomatouslobularmastitis,achronicinflammatorydiseaseofunknownetiology无枝菌酸棒杆菌